FastQCFastQC Report
Mon 21 Dec 2020
HV33TBGXG_n01_AKM176.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHV33TBGXG_n01_AKM176.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences13422235
Sequences flagged as poor quality0
Sequence length76
%GC38

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACGTCCGCACATCTCGTAT2673541.991873931576969TruSeq Adapter, Index 18 (97% over 40bp)

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTCCGC326250.064.2307933
CGTATGC332600.064.07139646
ACGTCCG331200.063.7450432
ATGCCGT332900.063.69987549
GTCCGCA328600.063.56926734
GTATGCC336550.063.39292547
TCCGCAC328250.063.3064435
TATGCCG335800.063.21206748
TGCCGTC335750.063.18048550
CTCGTAT336500.062.91679844
GCCGTCT334400.062.86028351
CACGTCC338200.062.82746531
CGCACAT329750.062.6988137
TCGTATG338900.062.61745545
CCGCACA332050.062.02272436
GTCACGT351950.061.61090529
AGTCACG356000.061.37987528
TCACGTC353300.060.90151630
CCGTCTT350750.060.03941752
GCACATC346600.059.9813138
Produced by FastQC (version 0.11.5)