FastQCFastQC Report
Mon 21 Dec 2020
HV33TBGXG_n01_AKM175.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHV33TBGXG_n01_AKM175.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences14094149
Sequences flagged as poor quality0
Sequence length76
%GC55

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACCCGTCCCGATCTCGTAT6348514.504358510755066TruSeq Adapter, Index 16 (97% over 40bp)

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CTCGTAT727300.067.3561244
TCGTATG738400.067.1607445
GTCCCGA715050.067.0030336
TCTCGTA730750.066.7124343
CGTATGC758000.065.86951446
CGTCCCG737750.065.76276435
CCGTCCC740400.065.6361434
TCCCGAT728000.065.4426737
ATCTCGT735150.065.1952942
GTATGCC766100.065.0545247
CCCGTCC753450.064.9592833
ACCCGTC762550.064.580532
GTCTTCT762700.064.5322454
CCGTCTT772300.063.9591852
TATGCCG776800.063.9029548
CAGTCAC804500.063.5156127
TCTGCTT758500.063.51218858
CCCGATC746000.063.29963738
GCCGTCT779700.063.27606251
TCTTCTG779150.063.22706655
Produced by FastQC (version 0.11.5)