FastQCFastQC Report
Mon 21 Dec 2020
HV33TBGXG_n01_AKM174.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHV33TBGXG_n01_AKM174.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences13262696
Sequences flagged as poor quality0
Sequence length76
%GC51

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACATGTCAGAATCTCGTAT4765953.593500145068544TruSeq Adapter, Index 15 (97% over 40bp)
GATCGGAAGAGCACACGTCTGAACTCCAGTCACATGTCAGAATCTCGGAT614430.4632768480857889TruSeq Adapter, Index 15 (97% over 40bp)
GATCGGAAGAGCACACGTCTGAACTCCAGTCACATGTCAGAATCTCGTTT420510.31706223229424846TruSeq Adapter, Index 15 (97% over 40bp)
GATCGGAAGAGCACACGTCTGAACTCCAGTCACATGTCAGAATCGCGTAT215050.16214651983277006TruSeq Adapter, Index 15 (97% over 40bp)
GATCGGAAGAGCACACGTCTGAACTCCAGTCACATGTCAGAATCTCGTGT148510.1119757249958832TruSeq Adapter, Index 15 (97% over 40bp)
GATCGGAAGAGCACACGTCTGAACTCCAGTCACATGTCAGAATCTCGGTT145180.10946492327050246TruSeq Adapter, Index 15 (97% over 40bp)
GATCGGAAGAGCACACGTCTGAACTCCAGTCACATGTCAGAATCGCGGAT137280.10350836662470436TruSeq Adapter, Index 15 (97% over 40bp)

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CTCGTAT552050.066.2391344
TCGTATG505000.065.7804445
TCTCGTA565400.065.40604443
CATGTCA878300.064.8247633
TGTCAGA875400.064.8238135
ATGTCAG876750.064.78812434
AGTCACA905600.064.5119528
CGTATGC438700.064.49003646
ACATGTC885750.064.44034632
GTCACAT898500.064.4390829
CACATGT890400.064.4281931
AATCTCG746700.064.3947241
TCACATG894000.064.3807130
ATCTCGT645650.064.1247142
GTCAGAA882300.064.0822836
CAGTCAC920900.063.70879427
GTATGCC449500.063.3931447
GAATCTC765050.063.12754440
CCAGTCA926550.062.97842426
AGAATCT799200.062.79816439
Produced by FastQC (version 0.11.5)