FastQCFastQC Report
Mon 21 Dec 2020
HV33TBGXG_n01_AKM173.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHV33TBGXG_n01_AKM173.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences13581892
Sequences flagged as poor quality0
Sequence length76
%GC38

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACAGTTCCGTATCTCGTAT8379236.169412921263105TruSeq Adapter, Index 14 (97% over 44bp)
GATCGGAAGAGCACACGTCTGAACTCCAGTCACAGTTCCGTATCGCGTAT145580.1071868337636612TruSeq Adapter, Index 14 (97% over 45bp)

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTATGC926550.066.94955446
GTATGCC931050.066.93947647
TATGCCG912850.066.9043548
ATGCCGT913800.066.83095649
CGTATCT955350.066.69160539
TCGTATG931900.066.664445
CTCGTAT937800.066.57867444
TGCCGTC918600.066.554450
CCGTATC982750.066.5440538
GCCGTCT893750.066.4978151
TCCGTAT991650.066.1166237
GTTCCGT991950.065.9644735
AGTTCCG993750.065.9260134
GTATCTC964800.065.717440
GTCACAG1027900.065.5798129
TCTCGTA956200.065.5676443
CCGTCTT914150.065.4613852
TTCCGTA1001050.065.4242736
CAGTCAC1050350.065.2037727
CTGCTTG789000.065.0695859
Produced by FastQC (version 0.11.5)