FastQCFastQC Report
Mon 21 Dec 2020
HV33TBGXG_n01_AKM172.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHV33TBGXG_n01_AKM172.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences14656615
Sequences flagged as poor quality0
Sequence length76
%GC39

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACAGTCAACAATCTCGTAT14203049.690532227257112TruSeq Adapter, Index 13 (97% over 40bp)

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTATGC1591150.067.4320746
TATGCCG1583650.067.38901548
GTATGCC1591950.067.3392747
ATGCCGT1586400.067.27022649
CTCGTAT1580400.067.1808544
TCGTATG1593250.067.1608745
TGCCGTC1590400.067.0860850
GCCGTCT1583450.066.97842451
CACAGTC1617450.066.8800631
GTCACAG1637300.066.6171829
TCTCGTA1597050.066.5795343
CTGCTTG1549750.066.459459
CCGTCTT1599600.066.43371652
AGTCAAC1606000.066.38833634
ACAGTCA1625700.066.3230632
CAGTCAC1672300.066.2323127
GTCAACA1609700.066.1506935
ATCTCGT1605550.066.0775442
AGTCACA1667850.066.0625328
CCAGTCA1676300.065.9759726
Produced by FastQC (version 0.11.5)