FastQCFastQC Report
Mon 21 Dec 2020
HV33TBGXG_n01_AKM171.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHV33TBGXG_n01_AKM171.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences16879464
Sequences flagged as poor quality0
Sequence length76
%GC40

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACCTTGTAATCTCGTATGC331120419.616760342626993TruSeq Adapter, Index 12 (100% over 50bp)
GATCGGAAGAGCACACGTCTGAACTCCAGTCACCTTGTAATGTCGTATGC452900.26831420713359144TruSeq Adapter, Index 12 (98% over 50bp)
GATCGGAAGAGCACACGTCTGAACTCCAGTCACCTTGTAATATCGTATGC191030.11317302492543603TruSeq Adapter, Index 12 (98% over 50bp)

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CTCGTAT3637000.068.4721142
GTATGCC3743250.068.45074545
CGTATGC3735000.068.4377244
TCGTATG3718450.068.37308543
TATGCCG3735300.068.36146546
GCCGTCT3686150.068.3248249
ATGCCGT3719100.068.3041647
TGCCGTC3708650.068.2103848
TGTAATC3657550.068.1748136
GTAATCT3641400.068.1513437
TCTCGTA3655200.068.14599641
CCGTCTT3705950.068.09579550
CTGCTTG3652050.068.0642557
CCTTGTA3751700.068.0485133
TAATCTC3644200.068.04504438
ACCTTGT3769800.067.8940332
ATCTCGT3669800.067.8881340
AATCTCG3663000.067.8624839
GTCACCT3809850.067.84415429
TGCTTGA3670650.067.843758
Produced by FastQC (version 0.11.5)