FastQCFastQC Report
Mon 21 Dec 2020
HV33TBGXG_n01_AKM170.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHV33TBGXG_n01_AKM170.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences16667899
Sequences flagged as poor quality0
Sequence length76
%GC42

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACGGCTACATCTCGTATGC246794314.806563202716791TruSeq Adapter, Index 11 (100% over 50bp)
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG732000.4391675279529832No Hit
GATCGGAAGAGCACACGTCTGAACTCCAGTCACGGCTACATATCGTATGC196240.11773529465231342TruSeq Adapter, Index 11 (98% over 50bp)

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTATGC2787950.068.5193244
GTATGCC2789000.068.4916145
TATGCCG2790300.068.4207346
TCGTATG2793050.068.38689443
CTCGTAT2764400.068.3283742
GCCGTCT2785450.068.2222849
ATGCCGT2799050.068.1094847
CCGTCTT2794850.067.98176650
CTGCTTG2785150.067.8834157
TGCCGTC2805950.067.8685748
TCTCGTA2771250.067.8548641
GCTACAT2741650.067.76816635
GGCTACA2756050.067.6666534
CTACATC2724000.067.6467736
TACATCT2731750.067.6369437
CGGCTAC2773300.067.558433
ACGGCTA2794250.067.5344432
TGCTTGA2805650.067.4865958
ATCTCGT2777450.067.47269440
CATCTCG2756500.067.4518339
Produced by FastQC (version 0.11.5)