FastQCFastQC Report
Mon 21 Dec 2020
HV33TBGXG_n01_AKM169.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHV33TBGXG_n01_AKM169.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences18098104
Sequences flagged as poor quality0
Sequence length76
%GC41

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACTAGCTTATCTCGTATGC198528110.969552390681367TruSeq Adapter, Index 10 (100% over 50bp)
GATCGGAAGAGCACACGTCTGAACTCCAGTCACTAGCTTATCGCGTATGC1021930.5646613590020259TruSeq Adapter, Index 10 (98% over 50bp)
GATCGGAAGAGCACACGTCTGAACTCCAGTCACTAGCTTATCTCGGATGC447210.24710323247120253TruSeq Adapter, Index 10 (98% over 50bp)
GATCGGAAGAGCACACGTCTGAACTCCAGTCACTAGCTTATCTCGTATTC409370.2261949649532349TruSeq Adapter, Index 10 (98% over 50bp)
GATCGGAAGAGCACACGTCTGAACTCCAGTCACTAGCTTATCTCGTTTGC266060.14700987462554088TruSeq Adapter, Index 10 (98% over 50bp)

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TATGCCG2234600.068.1636446
GTATGCC2278850.068.10396645
CGTATGC2262200.068.1034144
ATGCCGT2146350.068.0532847
CTCGTAT2205150.067.9721342
TCGTATG2175550.067.92798643
TGCCGTC2143650.067.89783548
GCCGTCT2115250.067.74448449
CACTAGC2608000.067.3884831
TAGCTTA2575950.067.3733534
CTGCTTG2108100.067.35725457
GCTTATC2534950.067.32371536
TCTCGTA2241400.067.3150341
ACTAGCT2594850.067.28355432
CCGTCTT2172250.067.25960550
CTTATCT2369250.067.2355837
CTAGCTT2590950.067.0292433
AGCTTAT2589300.066.9232435
TCACTAG2637050.066.9215630
ATCTCGT2302500.066.79133640
Produced by FastQC (version 0.11.5)