FastQCFastQC Report
Mon 21 Dec 2020
HV33TBGXG_n01_AKM168.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHV33TBGXG_n01_AKM168.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences19987277
Sequences flagged as poor quality0
Sequence length76
%GC40

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACGATCAGATCTCGTATGC278060313.911865032940703TruSeq Adapter, Index 9 (100% over 50bp)
GATCGGAAGAGCACACGTCTGAACTCCAGTCACGATCAGATATCGTATGC436050.21816378489175886TruSeq Adapter, Index 9 (98% over 50bp)
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG254080.12712086794014013No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TATGCCG3068500.068.41849546
CGTATGC3068900.068.398444
GTATGCC3070400.068.3536445
ATGCCGT3069200.068.3365747
CTCGTAT3019750.068.2398242
TCGTATG3079250.068.2291343
TGCCGTC3076650.068.1462948
GCCGTCT3079450.068.02476549
GATCTCG2999900.067.9749439
AGTCACG3104800.067.8814828
CTGCTTG3087050.067.81393457
CAGATCT2989300.067.7854637
CGATCAG3055350.067.77067633
CCGTCTT3090050.067.7655350
CACGATC3070200.067.764531
AGATCTC3000950.067.71207438
TCTCGTA3032900.067.6487141
GTCACGA3098200.067.62749529
ACGATCA3078500.067.46068632
ATCTCGT3030900.067.3817740
Produced by FastQC (version 0.11.5)