FastQCFastQC Report
Mon 21 Dec 2020
HV33TBGXG_n01_AKM167.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHV33TBGXG_n01_AKM167.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences15806633
Sequences flagged as poor quality0
Sequence length76
%GC41

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACACTTGAATCTCGTATGC12402887.846629955917874TruSeq Adapter, Index 8 (100% over 50bp)
GATCGGAAGAGCACACGTCTGAACTCCAGTCACACTTGAATCGCGTATGC839590.5311630883060295TruSeq Adapter, Index 8 (98% over 50bp)
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG576640.3648088748565238No Hit
GATCGGAAGAGCACACGTCTGAACTCCAGTCACACTTGAATCTCGGATGC502660.3180057384770052TruSeq Adapter, Index 8 (98% over 50bp)
GATCGGAAGAGCACACGTCTGAACTCCAGTCACACTTGAATCTCGTATTC338960.21444162080564533TruSeq Adapter, Index 8 (98% over 50bp)
GATCGGAAGAGCACACGTCTGAACTCCAGTCACACTTGAATCTCGTTTGC232220.14691300797582887TruSeq Adapter, Index 8 (98% over 50bp)
GATCGGAAGAGCACACGTCTGAACTCCAGTCACACTTGAATCGCGGATGC198320.12546631531205918TruSeq Adapter, Index 8 (97% over 45bp)

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATGCC1449600.067.8662145
CGTATGC1436750.067.75577544
TCGTATG1367550.067.650943
CTCGTAT1391300.067.6081742
TATGCCG1422000.067.48424546
GTCACAC1794500.067.093329
TCTCGTA1413500.067.0399941
ATGCCGT1343650.066.9848947
GCCGTCT1316250.066.83858549
CAGTCAC1830650.066.8066527
AGTCACA1822950.066.6389128
ACACTTG1790850.066.5422332
TGCCGTC1349750.066.4578148
CCAGTCA1839600.066.4247626
CCGTCTT1359700.066.3891650
CTGCTTG1335250.066.360157
CACTTGA1789900.066.3341233
ATCTCGT1470150.066.2813440
AATCTCG1548200.066.2288339
TCACACT1813300.066.1846330
Produced by FastQC (version 0.11.5)