FastQCFastQC Report
Mon 21 Dec 2020
HV33TBGXG_n01_AKM165.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHV33TBGXG_n01_AKM165.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences13675704
Sequences flagged as poor quality0
Sequence length76
%GC38

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACGCCAATATCTCGTATGC9089696.646597498746683TruSeq Adapter, Index 6 (100% over 50bp)

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTATGC1010050.067.8411244
GTATGCC1010850.067.7928645
TATGCCG1011300.067.7384846
ATGCCGT1014700.067.39889547
CTCGTAT1011350.067.3702642
GCCGTCT1012000.067.29162649
TCGTATG1018800.067.2746543
AGTCACG1031300.067.157928
GTCACGC1018350.067.145329
TGCCGTC1019100.066.97394648
TCACGCC1015050.066.8182430
CACGCCA1014900.066.7445731
CCGTCTT1022700.066.5670550
CTGCTTG1020450.066.35248657
CAGTCAC1048500.066.3404527
TCTCGTA1030300.066.20975541
GCCAATA1012400.066.1397134
TATCTCG1022850.066.1388339
CGCCAAT1014950.066.0908233
ACGCCAA1023450.065.9956432
Produced by FastQC (version 0.11.5)