FastQCFastQC Report
Mon 21 Dec 2020
HV33TBGXG_n01_AKM164.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHV33TBGXG_n01_AKM164.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences14151445
Sequences flagged as poor quality0
Sequence length76
%GC38

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACACAGTGATCTCGTATGC8309255.871661869158944TruSeq Adapter, Index 5 (100% over 50bp)
GATCGGAAGAGCACACGTCTGAACTCCAGTCACACAGTGATGTCGTATGC1258270.88914594940658TruSeq Adapter, Index 5 (98% over 50bp)
GATCGGAAGAGCACACGTCTGAACTCCAGTCACACAGTGATCGCGTATGC147500.10422963874007214TruSeq Adapter, Index 5 (98% over 50bp)

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTATGC1100800.067.7299244
GTATGCC1106650.067.6221545
TATGCCG1098650.067.5476446
TCGTATG1076450.067.42551443
ATGCCGT1089150.067.363547
CTCGTAT943950.067.3264442
GCCGTCT1079050.067.17065449
TGCCGTC1088450.067.1110848
GATCTCG962400.066.8089839
CACAGTG1162450.066.50690533
GTCACAC1175850.066.43352529
ACGTCTG1202250.066.403615
CAGTCAC1192050.066.39551527
CCGTCTT1100450.066.36381550
CTGCTTG1070400.066.3548957
ACACGTC1208050.066.2699213
AGTGATC995000.066.228936
CACGTCT1207700.066.1821714
CCAGTCA1193550.066.1373126
CGTCTGA1207100.066.1136116
Produced by FastQC (version 0.11.5)