FastQCFastQC Report
Mon 21 Dec 2020
HV33TBGXG_n01_AKM161.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHV33TBGXG_n01_AKM161.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences11578980
Sequences flagged as poor quality0
Sequence length76
%GC41

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACTGACCAATCTCGTATGC6400775.527922148583036TruSeq Adapter, Index 4 (100% over 50bp)
GATCGGAAGAGCACACGTCTGAACTCCAGTCACTGACCAATATCGTATGC156960.1355559816149609TruSeq Adapter, Index 4 (98% over 50bp)

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATGCC784500.066.0407745
CGTATGC784450.066.0407144
TCGTATG785000.066.0142643
CTCGTAT761300.065.91473442
CACACGT804250.065.9070612
ACACGTC804100.065.81521613
ACGTCTG802500.065.8126815
CACGTCT803600.065.7095314
GCACACG808800.065.49303411
TATGCCG791950.065.4204746
CAGTCAC806200.065.2971427
AGTCACT801800.065.28680428
CGTCTGA810150.065.1710516
GCCGTCT793300.065.0574549
GTCACTG800300.065.040829
TCTCGTA770550.064.8980441
GAACTCC809150.064.8705221
ACTCCAG807700.064.8421823
GATCGGA818600.064.785621
CACTGAC788200.064.7656731
Produced by FastQC (version 0.11.5)