FastQCFastQC Report
Mon 21 Dec 2020
HV33TBGXG_n01_AKM160.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHV33TBGXG_n01_AKM160.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences12816130
Sequences flagged as poor quality0
Sequence length76
%GC39

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACTTAGGCATCTCGTATGC8839566.897214681811124TruSeq Adapter, Index 3 (100% over 50bp)
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG184470.14393580589460314No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CACTTAG1038100.067.9225831
ACTTAGG1035450.067.8447132
CGTATGC996050.067.8187744
GTATGCC1001550.067.6731645
TATGCCG997850.067.5608846
TCGTATG987000.067.5538643
CTCGTAT986750.067.2631842
AGGCATC1015800.067.2450436
ATGCCGT995100.067.1444447
GGCATCT998850.067.0525737
TTAGGCA1039200.066.9370734
GCCGTCT985950.066.8649149
ACGTCTG1078850.066.8118215
TGCCGTC994900.066.7272948
ACACGTC1083750.066.6453513
TAGGCAT1039050.066.62331435
CAGTCAC1077750.066.60326427
CACGTCT1083100.066.565814
CGTCTGA1083300.066.5284616
CCAGTCA1077100.066.49267626
Produced by FastQC (version 0.11.5)