FastQCFastQC Report
Mon 21 Dec 2020
HV33TBGXG_n01_AKM159.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHV33TBGXG_n01_AKM159.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences17578933
Sequences flagged as poor quality0
Sequence length76
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACCGATGTATCTCGTATGC243945913.877173318767413TruSeq Adapter, Index 2 (100% over 50bp)
GATCGGAAGAGCACACGTCTGAACTCCAGTCACCGATGTATCGCGTATGC459840.26158584255369766TruSeq Adapter, Index 2 (98% over 50bp)
GATCGGAAGAGCACACGTCTGAACTCCAGTCACCGATGTATCTCGGATGC218020.12402345466587762TruSeq Adapter, Index 2 (98% over 50bp)
GATCGGAAGAGCACACGTCTGAACTCCAGTCACCGATGTATCTCGTATTC202470.11517763905238161TruSeq Adapter, Index 2 (98% over 50bp)

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CTCGTAT2645800.068.5415442
TCGTATG2644600.068.4406443
TCTCGTA2667000.068.2862441
GTATGCC2706550.068.1918145
CGTATGC2694750.068.1835144
ATCTCGT2698000.067.9671340
GTATCTC2722100.067.94578638
GCCGTCT2611900.067.90977549
CCGTCTT2636100.067.8241550
TATCTCG2724500.067.7490239
TATGCCG2700400.067.6769646
AAAAGGG2618350.067.60606470
TGTATCT2740400.067.5835837
ATGTATC2808500.067.5763436
CGATGTA2846950.067.46928434
CTGCTTG2564300.067.42335557
ATGCCGT2668450.067.4131947
GATGTAT2849150.067.3545235
TGCCGTC2660700.067.3060848
TGCTTGA2577200.067.23759558
Produced by FastQC (version 0.11.5)