Basic Statistics
Measure | Value |
---|---|
Filename | HV33TBGXG_n01_AKM158.fastq.gz |
File type | Conventional base calls |
Encoding | Sanger / Illumina 1.9 |
Total Sequences | 15446318 |
Sequences flagged as poor quality | 0 |
Sequence length | 76 |
%GC | 39 |
Per base sequence quality
Per tile sequence quality
Per sequence quality scores
Per base sequence content
Per sequence GC content
Per base N content
Sequence Length Distribution
Sequence Duplication Levels
Overrepresented sequences
Sequence | Count | Percentage | Possible Source |
---|---|---|---|
GATCGGAAGAGCACACGTCTGAACTCCAGTCACATCACGATCTCGTATGC | 1538351 | 9.959337882335454 | TruSeq Adapter, Index 1 (100% over 50bp) |
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG | 28150 | 0.1822440791391191 | No Hit |
GATCGGAAGAGCACACGTCTGAACTCCAGTCACATCACGATATCGTATGC | 21943 | 0.14205974524155207 | TruSeq Adapter, Index 1 (98% over 50bp) |
Adapter Content
Kmer Content
Sequence | Count | PValue | Obs/Exp Max | Max Obs/Exp Position |
---|---|---|---|---|
CGTATGC | 174005 | 0.0 | 68.04171 | 44 |
TATGCCG | 174020 | 0.0 | 68.013855 | 46 |
GTATGCC | 174110 | 0.0 | 67.98457 | 45 |
ATGCCGT | 174560 | 0.0 | 67.73838 | 47 |
TCGTATG | 174975 | 0.0 | 67.71008 | 43 |
CTCGTAT | 171620 | 0.0 | 67.63017 | 42 |
CACGATC | 166365 | 0.0 | 67.62534 | 36 |
TGCCGTC | 175210 | 0.0 | 67.453964 | 48 |
GATCTCG | 169295 | 0.0 | 67.43933 | 39 |
ACGATCT | 167570 | 0.0 | 67.409546 | 37 |
CGATCTC | 168715 | 0.0 | 67.290504 | 38 |
GCCGTCT | 175450 | 0.0 | 67.28831 | 49 |
TCACGAT | 170120 | 0.0 | 67.23243 | 35 |
ACGTCTG | 179435 | 0.0 | 67.182396 | 15 |
ACACGTC | 179995 | 0.0 | 67.12673 | 13 |
CTGCTTG | 175485 | 0.0 | 67.06694 | 57 |
CATCACG | 172940 | 0.0 | 67.046844 | 33 |
CAGTCAC | 178530 | 0.0 | 67.02832 | 27 |
TCCAGTC | 177960 | 0.0 | 67.021454 | 25 |
CCGTCTT | 176085 | 0.0 | 66.993965 | 50 |