FastQCFastQC Report
Mon 21 Dec 2020
HV33TBGXG_n01_AKM158.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHV33TBGXG_n01_AKM158.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences15446318
Sequences flagged as poor quality0
Sequence length76
%GC39

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACATCACGATCTCGTATGC15383519.959337882335454TruSeq Adapter, Index 1 (100% over 50bp)
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG281500.1822440791391191No Hit
GATCGGAAGAGCACACGTCTGAACTCCAGTCACATCACGATATCGTATGC219430.14205974524155207TruSeq Adapter, Index 1 (98% over 50bp)

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTATGC1740050.068.0417144
TATGCCG1740200.068.01385546
GTATGCC1741100.067.9845745
ATGCCGT1745600.067.7383847
TCGTATG1749750.067.7100843
CTCGTAT1716200.067.6301742
CACGATC1663650.067.6253436
TGCCGTC1752100.067.45396448
GATCTCG1692950.067.4393339
ACGATCT1675700.067.40954637
CGATCTC1687150.067.29050438
GCCGTCT1754500.067.2883149
TCACGAT1701200.067.2324335
ACGTCTG1794350.067.18239615
ACACGTC1799950.067.1267313
CTGCTTG1754850.067.0669457
CATCACG1729400.067.04684433
CAGTCAC1785300.067.0283227
TCCAGTC1779600.067.02145425
CCGTCTT1760850.066.99396550