FastQCFastQC Report
Thu 3 Dec 2020
HV2Y7BGXG_n01_wt-40_LPK-3R.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHV2Y7BGXG_n01_wt-40_LPK-3R.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences4355112
Sequences flagged as poor quality0
Sequence length76
%GC35

[FAIL]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGCGATA6950.058.4144
GGATAGT34950.057.979596
GCGCTAC4000.057.746775
GGATAGG28400.057.6728326
CGGGATA12900.057.5161674
GATAGGT32650.057.454017
CGGATAG14600.057.0516055
CAGATAG18150.055.341265
GGGATAG26950.054.8021285
GCGATAA16350.054.5841035
CGATAGT17000.054.34996
CTGATAG22100.053.684785
CTATAGG14200.053.4829256
GGTCCCC2100.053.329747
GATAGTC16550.053.077997
GCGATAG14850.052.791665
TGATAGG33800.052.7041556
TATAGGG25050.052.67117
ACGATAG12550.052.4273455
CGATATG26300.051.7651676
Produced by FastQC (version 0.11.5)