FastQCFastQC Report
Thu 3 Dec 2020
HV2Y7BGXG_n01_wt-40_LPK-2R.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHV2Y7BGXG_n01_wt-40_LPK-2R.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2840372
Sequences flagged as poor quality0
Sequence length76
%GC37

[FAIL]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGCGATA4950.059.3920444
CCCGTCC2600.057.8786910
GGATAGT23650.055.791036
CCCGATA4150.054.8175284
CGGATAG9550.054.6055875
GATAGGT23750.054.2269867
GGGATAG20050.053.9384275
GCGATAA11300.053.582365
CGCTACG2700.053.146456
GCGATAG10900.052.979965
CGATAGT11700.052.9470256
CCGCTAC2850.052.8053325
CAGATAG13850.051.8034025
GATAGTC10650.049.948827
GGATAGG23100.049.8468936
GATAGGG22200.049.8155987
TGATAGG21950.049.747846
GGTCCCC1200.049.579137
CGGGATA11650.049.569234
CGCTACC1850.049.187626
Produced by FastQC (version 0.11.5)