FastQCFastQC Report
Thu 3 Dec 2020
HV2Y7BGXG_n01_wt-40_LPK-1R.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHV2Y7BGXG_n01_wt-40_LPK-1R.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2708044
Sequences flagged as poor quality0
Sequence length76
%GC39

[FAIL]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTCCCC1550.058.70337
CGCGATA6050.057.2707334
CCCGTCC10750.056.3194610
TGATAGG23700.056.2638666
GCGGTCC1750.055.998055
CCGCTAC2800.054.9980855
GCGATAA9900.054.4425475
GATAGGA25900.054.18337
GATAGTC11000.054.085037
GCGGTAC2850.054.0332075
GGATAGG18550.053.771716
GATAGGG19600.053.7441567
GCGATAG10300.053.6874475
CGGATAG10200.053.527555
GATAGGT18650.053.1041457
GGATAGT19550.052.9904826
ACGATAG9650.052.9515235
TCCCGTC9400.052.8665969
CGTATAG7800.052.498175
CCCGATA4350.052.297034
Produced by FastQC (version 0.11.5)