FastQCFastQC Report
Thu 3 Dec 2020
HV2Y7BGXG_n01_wt-40_LPHK-3S.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHV2Y7BGXG_n01_wt-40_LPHK-3S.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1853151
Sequences flagged as poor quality0
Sequence length76
%GC44

[FAIL]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCGCCCG353.7180143E-969.992747
CCCCATG600.069.9908548
GCTACAC1550.067.734917
GACCGTC1200.067.074579
CTGATAC2650.066.030895
CCGATAC1800.064.160025
CCTCTAC1200.064.160025
ACGTACC600.064.1582810
CAGCTAC2850.063.8530275
CGGGTAC1100.063.629775
ACTGCCC1000.063.03598865
CGCTTTA1000.062.9934652
CGACCTC501.4551915E-1162.9917648
GTCTGTC1350.062.21409222
CGCATCA1300.061.9149813
GCCGATA3800.061.704134
CGCTAAC1250.061.5936166
CTAGTCC1250.061.591958
GCTGATA8850.061.293084
ACGTTAC800.061.2436523
Produced by FastQC (version 0.11.5)