FastQCFastQC Report
Thu 3 Dec 2020
HV2Y7BGXG_n01_wt-40_LPHK-3R.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHV2Y7BGXG_n01_wt-40_LPHK-3R.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3055409
Sequences flagged as poor quality0
Sequence length76
%GC41

[FAIL]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CCGCTAC3300.061.5110365
CAGATAG21750.061.4671555
CGCGATA6200.060.9626664
CTGATAG17750.059.939665
CGCTACA5100.059.015666
CGCTAAC2150.058.6007356
GCTAGCG16250.058.58077
GATAGTC10250.058.3863377
GGATAGG16900.057.984296
ATTATAG25400.057.732365
TTATAGC21100.057.5553936
CCTATAG9550.057.5354235
CGGATAG11850.057.2957465
CTATAGT12500.057.116186
ACGATAG12150.057.0332225
GGATAGC9800.056.781926
GGGATAG17200.056.7694635
GGATAGT17850.056.662886
CCTGATA8100.056.600234
CGATAGT12750.056.545246
Produced by FastQC (version 0.11.5)