FastQCFastQC Report
Thu 3 Dec 2020
HV2Y7BGXG_n01_wt-40_LPHK-2S.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHV2Y7BGXG_n01_wt-40_LPHK-2S.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1759132
Sequences flagged as poor quality0
Sequence length76
%GC41

[FAIL]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGATAGG15150.064.6839456
CCGCTAC1550.063.2233435
GGATAGT13350.062.9188966
CGGATAG8450.062.541945
GGGATAG12650.062.5272065
CAGATAG9150.061.96485
CGGGATA6000.061.8309254
CGTGATA6500.060.8437844
GCTACCG3050.060.8173037
CGTATAC2650.060.752355
CCTCGAC750.060.6643035
CGCGATA4750.060.4186974
GATAGGG13850.060.3947567
GATAGTC5800.060.3424767
CCTGATA5000.060.197654
GTGATAG11700.060.1258665
AGGATAG11200.059.997665
GATAGTG14900.059.896997
ATAGTGG9700.059.8945738
TGGATAG12900.059.68765
Produced by FastQC (version 0.11.5)