FastQCFastQC Report
Thu 3 Dec 2020
HV2Y7BGXG_n01_wt-40_LPHK-2R.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHV2Y7BGXG_n01_wt-40_LPHK-2R.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences4055435
Sequences flagged as poor quality0
Sequence length76
%GC40

[FAIL]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CAGATAG24250.060.0376135
CTGATAG23900.059.1596075
GCTAGCG17550.058.4280787
TGATAGG29900.057.588496
CGCTACA6800.057.12896
CGATAGT13900.056.9030386
GATAGTC14650.056.8552327
GGATAGG22800.056.4878166
CGGATAG13950.056.4482045
ACGATAG13850.056.3503885
CCGCTAC5000.055.9966165
GATAGGA34250.055.6886947
CCGATAA11000.055.678455
GCCGATA6800.055.5848774
GGATAGT23650.055.4934776
CCAGATA17000.055.379014
GATAGGT22850.055.29077
GCGATAG12450.055.0970735
CCTATAG11650.054.975225
CGGCTAC7450.054.9631235
Produced by FastQC (version 0.11.5)