FastQCFastQC Report
Thu 3 Dec 2020
HV2Y7BGXG_n01_wt-40_LPHK-1S.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHV2Y7BGXG_n01_wt-40_LPHK-1S.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2737075
Sequences flagged as poor quality0
Sequence length76
%GC36

[FAIL]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GCTACCC950.062.627257
CGATAGG8450.062.5423436
CCTGATA6950.062.4440084
CCGATAC1850.062.430395
CGCGATA6500.060.8441664
TCTACGG2550.060.3879787
CTGCGAC352.9781768E-759.9980435
CGATATG9100.059.613446
GGATAGT15150.059.3709956
CGTATAC3600.059.303625
GATAGTG19000.059.1274877
GGATAGG17300.059.0732156
TGCGATA6700.059.0279274
GCGATAA9800.058.926655
GTGATAG17450.058.3648575
GATAGGC6750.058.070067
GCCGATA4950.057.977914
CCTCGAC850.057.645185
CTGATAG10450.057.6057745
CGCTAGC2250.057.5536776
Produced by FastQC (version 0.11.5)