FastQCFastQC Report
Thu 3 Dec 2020
HV2Y7BGXG_n01_wt-40_LPHK-1R.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHV2Y7BGXG_n01_wt-40_LPHK-1R.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2425366
Sequences flagged as poor quality0
Sequence length76
%GC40

[FAIL]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCCCGTC10100.059.5973439
CCCGTCC11450.059.29472710
TGATAGG22750.059.0715836
CCGCTAC2500.058.7946855
CGGGATA7550.058.4053154
GGATAGG16150.058.075246
CGGATAG8950.057.8718645
GATAGGT17300.056.84347
CAGATAG12050.056.3434525
GATAGGA26000.056.2629857
CGATAGT8550.055.258166
GCCGATA6150.055.198264
CGCTACA3750.055.0616876
CCGGATA6150.054.6292084
ACGATAG7700.054.540525
GCGATAG8150.054.5349435
GATAGGG15500.054.1875347
GGGATAG14450.053.766765
GGATAGT16450.053.612176
CGTATAG6550.053.430285
Produced by FastQC (version 0.11.5)