FastQCFastQC Report
Thu 3 Dec 2020
HV2Y7BGXG_n01_wt-40_HPLK-3S.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHV2Y7BGXG_n01_wt-40_HPLK-3S.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2612764
Sequences flagged as poor quality0
Sequence length76
%GC38

[FAIL]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGATAGT16100.059.3439836
CGGATAG9400.059.198295
GATAGGT19100.056.436137
CGATAGG9150.055.8433726
GCGATAG9000.055.607515
GATAGTC9250.054.8613137
ACGATAG8000.054.6839565
CGGGATA9150.054.313424
CCGATAC1950.053.8426635
TGATAGG18150.053.7981726
CACGATA6850.053.6461564
CGATAGT10900.053.6203776
CCCGATA4800.053.225724
GATAGGA18950.052.8197987
CGATATG12700.052.634386
CGTATAG7400.052.49665
TGCGATA8200.052.4965974
CCCGTCC4950.052.3198410
GATAGTG19500.052.0479137
CAGATAG13000.051.958175
Produced by FastQC (version 0.11.5)