FastQCFastQC Report
Thu 3 Dec 2020
HV2Y7BGXG_n01_wt-40_HPLK-3R.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHV2Y7BGXG_n01_wt-40_HPLK-3R.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences5441791
Sequences flagged as poor quality0
Sequence length76
%GC40

[FAIL]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGATAGG33800.062.3343356
CTGATAG37300.061.7391475
CTATAGG23100.061.5122576
GATAGTC23650.060.376597
GCTAGCG25050.060.215627
GGGATAG32850.059.7683225
GGATAGT38650.059.673776
GATAGGT36250.059.6650547
CGGATAG20500.059.4112855
CAGATAG29500.059.081425
TGATAGG48700.059.073196
GTATAGG28950.058.7537086
TATAGGC29750.058.3491177
CGATAGT21500.058.2763256
GATAGGA47800.058.2074857
TTATAGC34650.057.9770626
CCTATAG15400.057.7240265
TGGATAG41300.057.708625
CCGCTAC4800.057.600925
ATAGTGC12650.056.714618
Produced by FastQC (version 0.11.5)