FastQCFastQC Report
Thu 3 Dec 2020
HV2Y7BGXG_n01_wt-40_HPLK-2S.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHV2Y7BGXG_n01_wt-40_HPLK-2S.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2283633
Sequences flagged as poor quality0
Sequence length76
%GC39

[FAIL]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGATAGT16350.059.9369056
CGATAGG6850.058.24636
CGGATAG8250.058.1193355
GTGATAG14650.056.3804365
GATAGGT16350.053.7256287
TGATAGG15600.053.6200686
CGGGATA8600.053.3122374
GCGATAA8800.053.293745
ACGATAG6050.053.2214245
GATAGTC9250.052.9677857
CAGATAG11350.052.421225
CCTGATA6700.052.2371184
CGCTACA2950.052.2017066
TATAGCG5700.052.1878747
CGCGATA5100.052.155184
CGATATG9950.052.058626
GATAGGA15300.051.923027
CTGATAG12550.051.870845
CGATAGT8000.051.1858526
ACGGATA7550.050.991734
Produced by FastQC (version 0.11.5)