FastQCFastQC Report
Thu 3 Dec 2020
HV2Y7BGXG_n01_wt-40_HPLK-2R.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHV2Y7BGXG_n01_wt-40_HPLK-2R.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences5339717
Sequences flagged as poor quality0
Sequence length76
%GC42

[FAIL]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CTGATAG34250.061.6159825
GCTAGCG28700.060.4834377
CAGATAG32650.060.026295
GGATAGG28750.059.6478656
TGATAGG44750.058.967816
TTATAGC37350.058.938186
GATAGTC19850.058.8874327
GTATAGG24250.058.882346
CTATAGG21400.058.8742526
CGGATAG18800.058.825545
CGTATAG14550.058.2088475
CCGCTAC6200.057.5764855
GGATAGC14600.057.530096
CCTGATA15600.057.431754
CCTATAG14400.057.3569685
GGATAGT28750.057.213266
GGGATAG26850.057.090855
GATAGGT29950.057.024267
CCAGATA20450.056.988554
CGATAGT17700.056.747316
Produced by FastQC (version 0.11.5)