FastQCFastQC Report
Thu 3 Dec 2020
HV2Y7BGXG_n01_wt-40_HPLK-1S.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHV2Y7BGXG_n01_wt-40_HPLK-1S.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2717629
Sequences flagged as poor quality0
Sequence length76
%GC36

[FAIL]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGCGATA5000.059.497254
CGATAGT10600.058.1115886
GGATAGT19650.057.8864256
CGGGATA9050.057.234924
GGGATAG15950.057.0506485
GCGATAG9300.056.4490055
CGATATG11850.055.2305266
CGGATAG8200.055.0584265
CGATAGG8700.054.308836
GATAGTC10350.054.104777
CTGATAG12900.053.4471365
CGTGATA10000.053.197544
GATAGGG15750.053.109637
TAACGCG407.492472E-752.495649
GCGGTAC1950.052.0488785
TGATAGG17500.051.7985576
GGATAGG17500.051.3985676
GATAGGT20800.051.152427
GGGATAA14550.050.99425
GTGATAG21300.050.936615
Produced by FastQC (version 0.11.5)