FastQCFastQC Report
Thu 3 Dec 2020
HV2Y7BGXG_n01_wt-40_HPLK-1R.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHV2Y7BGXG_n01_wt-40_HPLK-1R.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences4460623
Sequences flagged as poor quality0
Sequence length76
%GC42

[FAIL]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CTGATAG30100.061.9721575
TGATAGG39000.060.7519156
TTATAGC32300.060.1348726
GGATAGG26400.060.0523726
CCGATAA11750.059.272215
CTCCCCG3250.059.2257278
GATAGGT24900.059.171677
CGGATAG15150.059.137545
GATAGTC17650.058.6918837
GATAAGC22800.058.328367
GGATAGT22900.057.921486
CTATAGG21000.057.8290566
TATAGGC25650.057.7143827
GTATAGG21400.057.5658346
GATAGGA39950.057.5545467
CAGATAG25600.057.5543375
CGCGATA8150.057.112344
TCTAGCG21950.057.079427
CGCTACG5900.056.9449426
GGGATAG22000.056.7912565
Produced by FastQC (version 0.11.5)