FastQCFastQC Report
Thu 3 Dec 2020
HV2Y7BGXG_n01_wt-40_HPK-3S.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHV2Y7BGXG_n01_wt-40_HPK-3S.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2184466
Sequences flagged as poor quality0
Sequence length76
%GC37

[FAIL]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGCGATA4800.056.8723454
GGATAGT14350.056.8266146
GCTAGCC1900.055.258047
CGGATAG6250.054.8774385
CGATAGG5950.054.7033276
CCTGATA5700.054.6465644
ACGATAG5950.054.115125
CGATAGT8200.053.3511626
GCGATAG7450.053.0847665
GCGGTCC1800.052.4975435
GTCCGCA800.052.4963468
GTGATAG15800.051.3900035
ACGGATA6200.051.3685654
CAGATAG9350.051.281025
GGGGTAC2800.051.2476045
GGGTAGC3350.051.191646
CGGGATA6850.050.581584
GATAGTG17600.050.506697
TGATAGG15500.050.352486
GGGATAG13300.050.2608075
Produced by FastQC (version 0.11.5)