FastQCFastQC Report
Thu 3 Dec 2020
HV2Y7BGXG_n01_wt-40_HPK-3R.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHV2Y7BGXG_n01_wt-40_HPK-3R.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3210534
Sequences flagged as poor quality0
Sequence length76
%GC42

[FAIL]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGATAGG18050.059.3330156
GCTAGCG14350.059.264297
CAGATAG21800.058.5987785
CTGATAG18050.058.363525
CGGATAG11350.058.279815
CCTGATA9850.057.9166684
CCGCTAC4050.057.899125
GCCGATA6550.057.707824
GATAGGT18050.057.392247
ACGATAG10750.056.9746675
CGTATAC4000.056.872935
GATAGTC12050.056.6351747
CTATAGG12800.056.5994956
GGGATAG16450.056.593685
GGATAGT19750.056.5295836
TATAGGC16550.056.2499777
CGATAGT11050.056.0613026
CGCTACA5450.055.869526
TGATAGG22500.055.8424036
GATAGGG19150.055.7403567
Produced by FastQC (version 0.11.5)