FastQCFastQC Report
Thu 3 Dec 2020
HV2Y7BGXG_n01_wt-40_HPK-2S.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHV2Y7BGXG_n01_wt-40_HPK-2S.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2383273
Sequences flagged as poor quality0
Sequence length76
%GC39

[FAIL]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGGGATA8250.056.845014
GCGATAG8400.055.413285
CGCGATA5200.055.188934
CGGATAG8250.055.1481445
GGATAGT15900.055.0280846
GCTAGCC2550.054.8974537
CGATAGG8750.054.3966756
CCTGATA6250.054.316684
GCGGTAC2200.054.0876055
GCGCTAC2400.053.9550365
GATAGTC8250.053.450167
CGATAGT8950.053.1811036
GATAGGG16050.052.98637
GATAGGT15550.052.8895457
GCGATAA8150.052.8188555
TGATAGG16450.052.549986
CGCTACA3150.052.2190326
GCTGATA12950.052.1589744
CGGCTAC3550.051.264475
CCCGTCC4600.050.97300710
Produced by FastQC (version 0.11.5)