FastQCFastQC Report
Thu 3 Dec 2020
HV2Y7BGXG_n01_wt-40_HPK-2R.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHV2Y7BGXG_n01_wt-40_HPK-2R.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2772434
Sequences flagged as poor quality0
Sequence length76
%GC38

[FAIL]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GCGACCG950.058.942397
GGATAGT22500.057.2396056
CGGATAG10050.056.7613685
CCCGTCC6500.055.99526610
GGATAGG18100.055.492556
CCGCTAC2150.055.3441625
GATAGTC11600.054.9091537
GATAGGT21050.054.8647237
CGCGATA5000.054.5953834
CTGATAG14900.054.257165
CGGGATA9200.054.017174
CAGATAG10900.053.298255
TGATAGG24800.052.636686
GCGATAG10800.051.8474735
CCTGATA7500.051.3294
CGATAGT12700.051.2555126
CCCGATA3900.051.1495254
GATAGGA23150.050.946027
GGGATAG17900.050.833695
GATAGGG20600.050.626797
Produced by FastQC (version 0.11.5)