FastQCFastQC Report
Thu 3 Dec 2020
HV2Y7BGXG_n01_wt-40_HPK-1S.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHV2Y7BGXG_n01_wt-40_HPK-1S.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2460578
Sequences flagged as poor quality0
Sequence length76
%GC38

[FAIL]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGCGATA4550.062.3073124
CGATAGT9750.058.1534926
ACGATAG7700.057.2723775
CGATAGG8650.057.0516746
GGATAGT16100.056.7387856
GATAGTG20300.056.2031257
CGGATAG8650.055.837815
CAGATAG10500.055.3329965
GATAGTC10300.054.705077
CGGGATA7650.054.4441154
GCGATAG8050.054.3474965
GATAGGT19250.053.8145757
CGTGATA10700.053.3174324
GCGATAA9250.052.972655
GATAGGG18000.052.6909147
CCAGATA6250.052.639684
GTGATAG19100.052.224815
GGGGTAC3300.051.969385
CGATATG11750.051.53166
GATAGGA17950.051.472877
Produced by FastQC (version 0.11.5)