FastQCFastQC Report
Thu 3 Dec 2020
HV2Y7BGXG_n01_wt-40_HPK-1R.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHV2Y7BGXG_n01_wt-40_HPK-1R.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2819089
Sequences flagged as poor quality0
Sequence length76
%GC38

[FAIL]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CCCGTCC6050.060.16010710
CGGATAG10200.059.358695
CGCGATA6150.057.4757464
GGATAGT18650.055.733456
GGATAGG18100.054.5266536
CGGGATA9750.053.842474
CCGCTAC3150.053.329685
GATAGGG20400.053.011087
GCGATAA10950.052.736125
GATAGGT20150.052.626677
TGATAGG23050.052.5343676
GCGATAG11500.052.3442425
CGCTACC2750.052.178256
CCTGATA9550.051.305394
CAGATAG13750.050.905615
CGATAGT11950.050.665996
CTATAGG9750.049.8940246
CGATAAA13550.049.8489886
GGGATAG20050.049.3981175
ACGATAG7300.049.3801845
Produced by FastQC (version 0.11.5)