FastQCFastQC Report
Thu 3 Dec 2020
HV2Y7BGXG_n01_wt-10_LPK-6S.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHV2Y7BGXG_n01_wt-10_LPK-6S.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences5404925
Sequences flagged as poor quality0
Sequence length76
%GC48

[FAIL]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[WARN]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CCGATAC6100.066.5548255
CTGATAC7400.065.740725
GATAGTC20900.065.643427
CGATACA12350.065.4636846
GGCTACC6150.064.876166
CTGCTAC5450.064.85995
CGATATG18600.064.72936
TGCTACC2550.064.5079356
GGATAGT20650.064.404916
CCGCTAC4600.063.910595
CTTCTAC6300.063.8864365
CTGATAA29050.063.8529785
GCGATAC7850.063.755525
CCGATAA21600.063.6781165
TGATAGT18750.063.4648176
CTCTACA7700.063.179986
GGATATG22200.063.061236
TCTACAC5500.062.997597
CCTCTAC5500.062.997595
GGATAGG34050.062.8028766
Produced by FastQC (version 0.11.5)