FastQCFastQC Report
Thu 3 Dec 2020
HV2Y7BGXG_n01_wt-10_LPK-5S.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHV2Y7BGXG_n01_wt-10_LPK-5S.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2818198
Sequences flagged as poor quality0
Sequence length76
%GC37

[FAIL]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGGATAG10750.058.92855
CGCGATA6200.058.143454
CGATAGG11050.057.9620976
GGATAGG19400.057.9106676
GATAGTC8100.057.8985067
CCCGATA4950.056.5639954
CGTGATA9350.056.5224044
GTGATAG17000.056.204235
GGATAGT16450.055.5302856
CAGATAG12900.055.3472145
GATAGGT19100.055.1544727
GATAGGG21200.055.1389127
CCAGATA9550.054.972214
CGATATG8200.054.632546
ACGATAG8850.054.574675
CGATAGT9500.054.524716
GCCGATA6250.054.31844
TGATAGG18300.054.3153426
GCGATAA10150.054.136345
ACGGATA9700.054.122124
Produced by FastQC (version 0.11.5)