FastQCFastQC Report
Thu 3 Dec 2020
HV2Y7BGXG_n01_wt-10_LPK-4S.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHV2Y7BGXG_n01_wt-10_LPK-4S.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3187613
Sequences flagged as poor quality0
Sequence length76
%GC45

[FAIL]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CTGATAC4650.063.97685
GGATAGT14150.063.0725366
GATAGTC10050.062.336667
GATAGTT14500.062.2742167
CGATACA7900.062.0236746
CCGATAC4500.061.442825
TGATAGG16350.061.4356846
GCGGATA14500.060.8259774
CGCTAAC1900.060.787866
GCGATAA14400.060.7622875
ACGATAC4450.060.5601965
CGATATG8850.060.1114046
CGATAGG11800.059.913676
CGCTACA5650.059.4674536
CGTCGAT4650.059.4598583
TCTACCG3600.059.3039937
GATATGG14600.059.2107627
CGATAAA34450.058.822836
GCGATAG13150.058.8197365
CTGATAA16350.058.652885
Produced by FastQC (version 0.11.5)