FastQCFastQC Report
Thu 3 Dec 2020
HV2Y7BGXG_n01_wt-10_LPK-3S.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHV2Y7BGXG_n01_wt-10_LPK-3S.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3267201
Sequences flagged as poor quality0
Sequence length76
%GC40

[FAIL]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGGATAG11900.060.586385
GGATAGG25850.059.844356
GATAGGG27400.058.6295977
TGATAGG24550.058.451246
CGGGATA11750.058.38034
GATAGTC12350.058.378787
GGATAGT20350.058.3037726
GGGATAG23650.058.0109065
GCTAGCG6050.057.849477
CGTGATA10900.057.4744224
GCGATAA14150.057.3834045
CGCGATA8150.057.113944
GCGATAG11950.056.8183485
ACGGATA9600.056.507824
CAGATAG16450.056.3812525
GCCGATA6350.056.217894
GATAGGC14900.055.9043277
CGATAGG11850.055.8219266
CCTGATA7150.055.8016364
CGATAGT11250.055.6880386
Produced by FastQC (version 0.11.5)