FastQCFastQC Report
Thu 3 Dec 2020
HV2Y7BGXG_n01_wt-10_LPK-2S.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHV2Y7BGXG_n01_wt-10_LPK-2S.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3432687
Sequences flagged as poor quality0
Sequence length76
%GC39

[FAIL]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GCGATAA14150.063.5664525
CCTGATA10500.062.9975784
GGATAGG24800.062.9420436
CGGATAG13500.062.4790765
CCGATAA14150.061.835075
CAGATAG14800.061.4841235
CCGATAC2850.061.4011465
GATAGTC11900.061.170557
GGGATAG21150.060.8965
GTCGATA12650.060.867224
CTGATAG17850.060.7819755
CGATAGT10050.060.247316
CGATAAA40900.060.2430576
CGATAGG12750.060.1162076
GCGATAG10600.060.0920265
CGCTAAC2100.059.998566
GATAGTG19750.059.715747
TGATAGG23950.059.622796
GCTGATA16850.059.6119464
GTGATAG18650.059.4883235
Produced by FastQC (version 0.11.5)