FastQCFastQC Report
Thu 3 Dec 2020
HV2Y7BGXG_n01_wt-10_LPK-1S.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHV2Y7BGXG_n01_wt-10_LPK-1S.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2930382
Sequences flagged as poor quality0
Sequence length76
%GC51

[FAIL]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CCTCTAC3750.066.262945
TTCTACC2950.065.2505656
GGGCTAC2850.065.084055
CTGATAG9050.064.968715
CTGATAA14900.064.593675
GCGATAA14700.063.5678445
GTGCTAC1600.063.4339265
TGCTACC1550.063.2222446
CGATATG7200.063.1908876
AATCTAC4100.063.167185
TCGATAC3350.062.6830375
CGATAGG11950.062.6742946
CTCTACA4300.062.670896
CGATAAA29550.062.6529856
TGATAGT8500.062.584716
TCGATAA13500.062.4779625
CGATACA6000.062.413156
GATAAAC7250.062.271297
GATAGTT9250.062.04957
GATAATC7800.061.9185337
Produced by FastQC (version 0.11.5)