FastQCFastQC Report
Thu 3 Dec 2020
HV2Y7BGXG_n01_wt-0.5_LPK-3S.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHV2Y7BGXG_n01_wt-0.5_LPK-3S.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2824166
Sequences flagged as poor quality0
Sequence length76
%GC38

[FAIL]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CACGATA7800.063.2667354
CGGATAG8850.061.2970165
CCTCGAC700.059.997635
CGATAAA34050.059.9242136
CGATAGG9300.059.8363536
CGGGATA9150.058.9047784
ACGATAG8700.058.733325
CGCGATA6750.058.5902824
CGCTACA3300.058.3310286
CCGATAA12100.057.8489575
GCGATAA13200.057.8007475
CAGATAG12300.057.477415
TGCTACC1650.057.2704666
GATAGGT15000.057.16347
GGATAGT13600.057.1300966
AGGATAG13550.057.082625
CCGCTAC2950.056.9469035
CGATAGT7500.056.9310876
CTACACG1050.056.6624268
CGGATAA14900.056.3736155
Produced by FastQC (version 0.11.5)