FastQCFastQC Report
Thu 3 Dec 2020
HV2Y7BGXG_n01_wt-0.5_LPK-3R.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHV2Y7BGXG_n01_wt-0.5_LPK-3R.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences4530582
Sequences flagged as poor quality0
Sequence length76
%GC39

[FAIL]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGATAGG24850.059.8555036
CTGATAG26050.059.785225
CAGATAG26050.058.710435
CGGATAG14450.058.6123435
GATAGGT26700.058.5906377
GATAGTC17300.058.2610557
CTACGCG7800.057.8791668
TGATAGG36050.056.792656
CGATAGT18450.056.5277146
CGCGATA7600.056.180784
CTATAGG16000.055.777856
GCTAGCG19750.055.463757
GGATAGT28550.055.0404246
GATAGGA37250.054.9618767
GCGATAG14400.054.4411245
GGGATAG22600.054.3550875
CCGATAA12000.054.246695
CCTGATA12850.054.1990284
ACGATAG15650.054.1181075
GGATAGC11650.054.073956
Produced by FastQC (version 0.11.5)