FastQCFastQC Report
Thu 3 Dec 2020
HV2Y7BGXG_n01_wt-0.5_LPK-2S.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHV2Y7BGXG_n01_wt-0.5_LPK-2S.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3186370
Sequences flagged as poor quality0
Sequence length76
%GC33

[FAIL]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GATAGCC2800.061.2435467
CGATAGG10150.058.6145136
CGCGATA5350.057.5640264
GGTCCCC1400.057.4939427
CCCGATA4150.057.3433574
CGGATAG11200.057.1814775
CCGATAA9650.056.574355
GGATAGG23650.055.9349146
GCTGATA15850.055.861624
GATAGGT20800.055.8594977
GATAGGG22950.055.8111157
CGGGATA10300.055.7222824
TGATAGG19900.055.5720336
GCGATAA12550.055.4921575
CGATATG10250.055.3112456
GGATAGT20100.055.1931886
GCGATAG10350.055.1149675
CCTGATA7250.055.0286834
TGGATAG21650.054.9595685
TATAGCG7600.054.796867
Produced by FastQC (version 0.11.5)