FastQCFastQC Report
Thu 3 Dec 2020
HV2Y7BGXG_n01_wt-0.5_LPK-1S.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHV2Y7BGXG_n01_wt-0.5_LPK-1S.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3121273
Sequences flagged as poor quality0
Sequence length76
%GC32

[FAIL]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GCCGATA6950.059.419664
CACGATA7650.058.099884
GCGATAG10050.057.806285
CGATAGT10250.057.7026526
GGATAGG20350.057.6120536
GGGATAG18100.057.039595
GGATAGT19000.056.9164546
ACGATAG8950.056.699335
CGCGATA6300.056.662094
CGGATAG10700.056.257145
GCGGATA9600.055.7767454
GATAGGG18600.055.691757
GCGGTCC1650.055.147065
GATAGGT19950.054.729797
GATAGTC9750.054.557077
CGATATG12350.054.4085586
CCTGATA8350.054.067494
CGATAGG10050.053.975746
GCGATAA12300.053.7761425
CGGGATA10900.053.6195224
Produced by FastQC (version 0.11.5)