FastQCFastQC Report
Thu 3 Dec 2020
HV2Y7BGXG_n01_wt-0.5_LPK-1R.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHV2Y7BGXG_n01_wt-0.5_LPK-1R.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2484239
Sequences flagged as poor quality0
Sequence length76
%GC40

[FAIL]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTATAC2900.061.5485885
GATAGGA39250.060.72187
TGATAGG32750.059.844286
GTAGGAC10150.059.649748
TCCCGTC10200.059.3573389
CGCTACC2800.058.747016
CGGATAG9050.058.3948175
CGCGATA5950.058.2323274
GGATAGG16550.057.308266
CGGCTAC4000.056.872115
CCGCTAC2600.056.5355835
CTAGGAC10850.056.446478
GCGATAA7700.056.3607645
GATAGTC12050.056.3445437
GGATAGT16300.056.2548036
GTCCCGT4000.055.99498
CCCGTCC12700.055.9397810
CAGATAG12100.055.8236055
GCTACCG7050.055.5988927
CGATAGT10000.055.2971886
Produced by FastQC (version 0.11.5)