FastQCFastQC Report
Thu 3 Dec 2020
HV2Y7BGXG_n01_wt-0.5_LPHK-3S.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHV2Y7BGXG_n01_wt-0.5_LPHK-3S.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2663736
Sequences flagged as poor quality0
Sequence length76
%GC35

[FAIL]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGCGATA4500.057.552444
CCTGATA5750.056.605634
GATAGGT20700.054.947687
CGATAGG8600.054.9388856
CACGATA6800.054.041194
GCGATAA8500.053.938255
CGGATAG7800.053.8432355
GGATAGT17950.053.6182676
GGATAGG19050.053.461676
TGATAGG19650.053.0760086
CCCGATA3100.053.0616384
CAGATAG11950.053.009685
GGGATAG18150.052.83465
GTGATAG17400.052.6982965
GATAGGG18600.052.684337
GCGATAG9000.052.4971585
GCGGTCC2200.052.4971545
ACGGATA7900.052.275654
CGGTAGG3950.052.275656
GCCGATA4500.052.108294
Produced by FastQC (version 0.11.5)