FastQCFastQC Report
Thu 3 Dec 2020
HV2Y7BGXG_n01_wt-0.5_LPHK-3R.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHV2Y7BGXG_n01_wt-0.5_LPHK-3R.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2931204
Sequences flagged as poor quality0
Sequence length76
%GC38

[FAIL]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGATAGG19450.056.1414576
CGGATAG9450.055.923435
CCCGTCC6850.055.6890510
GATAGGT20350.054.861487
CAGATAG12950.054.3219035
CCGCTAC3500.053.9975935
CGATAGT12400.053.626646
CGCTACG3200.053.591366
CCTATAG5500.053.4521645
CCCGATA4000.053.372624
CGGGATA9250.053.3489764
GGATAGT21000.052.830986
CGCGATA6500.052.7668764
TGATAGG25450.052.394526
GATAGTC12200.051.923037
GATAGGA26450.051.8682567
GCGATAG12100.051.485315
GATAGGG19350.051.3663447
CCTGATA9550.050.5736584
GGGATAG19900.050.1233945
Produced by FastQC (version 0.11.5)